P17-21 Development of novel nanotoxicity assessment method utilizing 3D printing system
نویسندگان
چکیده
Superoxide dismutase (SOD) is the first line of defense against oxidative damage. Malathion an organophosphate insecticide and can induce production reactive oxygen species (ROS) cause intracellular stress. The present study was undertaken to examine effects malathion on SODs activity their transcriptional levels in Oxya chinensis (Thunberg) (Orthoptera: Acrididae). results showed that total SOD MnSOD activities increased as a dose-dependent manner while CuZnSOD has no significant changes after treatments. Total were highest at concentration 0.8 μg μL−1 treatment significantly about 1.81- 2.48-fold compared with control, respectively. Increased mRNA expression MnSOD, ecCuZnSOD1, ecCuZnSOD2 observed Moreover, alteration transcript similar profiles activity. These suggested up-regulation led increase order eliminate excessive ROS caused by malathion. In addition, we evaluated role individual gene stress using RNAi recombinant proteins. contents silencing ecCuZnSOD1 genes. OD values E. coli cells transformed pET-28a-OcMnSOD plasmid 1.13–1.31-fold 1.08–1.33-fold higher than those pET-28a plasmids under 0.4 treatments, findings indicated exerted important
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ژورنال
عنوان ژورنال: Toxicology Letters
سال: 2022
ISSN: ['0378-4274', '1879-3169']
DOI: https://doi.org/10.1016/j.toxlet.2022.07.631